Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: Tooth development is independent of a Hox patterning programme.

doi: 10.1002/dvdy.10168

Figure Lengend Snippet: Fig. 3. Hoxa2 expression is maintained in second arch with ectopic fibroblast growth factor 8 (FGF8). FGF8 beads were implanted into explants of second arch mesenchyme at embryonic day 10.5. Second arches were cultured for 24 hr. A: Lightfield photomicrograph. B: Section adjacent to A showing radioactive in situ hybridization for Hoxa2 expres- sion in second arch mesenchyme surrounding FGF8 bead. The FGF8- soaked bead is outlined in red.

Article Snippet: Bead Implants Heparin-coated acrylic beads were rinsed in phosphate buffered saline (PBS) then incubated overnight in 1 mg/ml recombinant mouse FGF8 protein (R&D Systems) at 4°C.

Techniques: Expressing, Cell Culture, In Situ Hybridization

Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: Tooth development is independent of a Hox patterning programme.

doi: 10.1002/dvdy.10168

Figure Lengend Snippet: Fig. 4. Comparative analysis of expression of Hoxa2 and Fgf8 in head and neck of embryonic day 10.5 mouse embryos. In situ hybridisa- tion (with 35S-labeled riboprobes) on serial sections of the embryos. A: Fgf8 expression in caudal second arch epithelium (arrow). B: Hoxa2 expression in caudal second arch epithelium in which Fgf8 is expressed (arrow). Mx, maxillary processes; Ma, mandibular processes; II, second arch.

Article Snippet: Bead Implants Heparin-coated acrylic beads were rinsed in phosphate buffered saline (PBS) then incubated overnight in 1 mg/ml recombinant mouse FGF8 protein (R&D Systems) at 4°C.

Techniques: Expressing, In Situ, Labeling

Journal: Oncotarget

Article Title: FGF8 promotes colorectal cancer growth and metastasis by activating YAP1

doi:

Figure Lengend Snippet: QRT-PCR (A) and imunoblot (B) analysis of FGF8 level in human CRC tissues (T) and adjacent normal mucosa tissues (N) from the same patient. All data were from at least three independent experiments. *, P<0.05; **, P<0.01.

Article Snippet: FGF8 recombinant protein was purchased from Protech and used at 250 ng/ml.

Techniques: Quantitative RT-PCR

Journal: Oncotarget

Article Title: FGF8 promotes colorectal cancer growth and metastasis by activating YAP1

doi:

Figure Lengend Snippet: (A) Immunohistochemical staining of FGF8 in tumor and corresponding colorectal mucosa. (B) Immunohistochemical scores for FGF8 in normal colorectal mucosa and CRC tissues. (C) Expression of FGF8 in the primary tumors without (N0) or with (N1/N2) lymph node metastasis was analyzed. Left, overall tumors; middle, stage T1–T2; right, stage T3–T4. (D) Kaplan–Meier survival curves of CRC patients with low (n = 34) and high (n = 38) FGF8 expression. Left, overall tumors; middle, stage T1–T2; right, stage T3–T4. ***, P<0.001.

Article Snippet: FGF8 recombinant protein was purchased from Protech and used at 250 ng/ml.

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Oncotarget

Article Title: FGF8 promotes colorectal cancer growth and metastasis by activating YAP1

doi:

Figure Lengend Snippet: RKO cells were treated with or without FGF8 and/or PD173074. (A) Representative photographs of colony formation 14 days after culture of cells. (B) Proliferation rate as measured by BrdU labeling for 12 hours. (C) Quantitative analysis of cell migration and matrigel invasion assays. Migration was analyzed at 24 h, and invasion at 48 h. (D) Representative phase-contrast images of RKO cell morphology. (E) Expression of Snail, E-cadherin and Vimentin was examined by immunoblot. All data were from at least three independent experiments. **, P<0.01; ***, P<0.001.

Article Snippet: FGF8 recombinant protein was purchased from Protech and used at 250 ng/ml.

Techniques: Labeling, Migration, Expressing, Western Blot

Journal: Oncotarget

Article Title: FGF8 promotes colorectal cancer growth and metastasis by activating YAP1

doi:

Figure Lengend Snippet: (A) Mean tumor volume and Ki67 expression in tumors after subcutaneous transplantation of RKO-FGF8 or RKO-mock cells. (B) Histopathology showing the lung metastases in mice and quantification of the lung weight and number of metastases following tail-vein injection with RKO-FGF8 or RKO-mock cells. **, P<0.01; ***, P<0.001.

Article Snippet: FGF8 recombinant protein was purchased from Protech and used at 250 ng/ml.

Techniques: Expressing, Transplantation Assay, Histopathology, Injection

Journal: Oncotarget

Article Title: FGF8 promotes colorectal cancer growth and metastasis by activating YAP1

doi:

Figure Lengend Snippet: (A) We identified hub proteins implicated in core pathways according to the following four gold standards: degree, link, module and microarray analyses. (B) The global PPI network. (C) The core sub-network in cancer. (D) Identification of FGF8-YAP1 interaction in CRC cells.

Article Snippet: FGF8 recombinant protein was purchased from Protech and used at 250 ng/ml.

Techniques: Microarray

Journal: Oncotarget

Article Title: FGF8 promotes colorectal cancer growth and metastasis by activating YAP1

doi:

Figure Lengend Snippet: (A) Immunoblot analysis of cytoplasmic and nuclear YAP1 in RKO cells treated with or without FGF8 or/and PD173074. β-Actin was used as a cytoplasmic protein loading control, and histone-3 (H3) was used for nuclear protein loading control. (B) MRNA level CTGF and CYR61 was examined by qRT-PCR. (C) Transcription activity of TEAD4 was examined by luciferase assay. (D) Expression of YAP1 and FGF8 in serial human colorectal tumor sections was examined by immunohistochemical staining. (E) Correlation between the expression levels of YAP1and FGF8. (F) Expression of YAP1 in high-FGF8-expressing tumors and low-FGF8-expressing tumors was analyzed. All data were from at least three independent experiments.*, P<0.05; **, P<0.01.

Article Snippet: FGF8 recombinant protein was purchased from Protech and used at 250 ng/ml.

Techniques: Western Blot, Control, Quantitative RT-PCR, Activity Assay, Luciferase, Expressing, Immunohistochemical staining, Staining

Journal: Oncotarget

Article Title: FGF8 promotes colorectal cancer growth and metastasis by activating YAP1

doi:

Figure Lengend Snippet: FGF8-treated RKO cells were transfected with siYAP1 or siNC. (A-B) Proliferative activity was measured by a colony formation assay (A) and BrdU labeling (B). (C) Cell migration and invasion was examined by transwell assay and matrigel invasion assays. (D) Representative phase-contrast images of RKO cell morphology. (E) Expression of Snail, E-cadherin and Vimentin was examined by immunoblot. All data were from at least three independent experiments. *, P<0.05; **, P<0.01; ***, P<0.001.

Article Snippet: FGF8 recombinant protein was purchased from Protech and used at 250 ng/ml.

Techniques: Transfection, Activity Assay, Colony Assay, Labeling, Migration, Transwell Assay, Expressing, Western Blot

Journal: Oncotarget

Article Title: FGF8 promotes colorectal cancer growth and metastasis by activating YAP1

doi:

Figure Lengend Snippet: QRT-PCR analysis of YAP1 mRNA level in FGF8-treated RKO, SW480 and HCT116 cells in the present or absent of PD173074.

Article Snippet: FGF8 recombinant protein was purchased from Protech and used at 250 ng/ml.

Techniques: Quantitative RT-PCR